Recently, Li Mingyou and Guilang research team from the College of Fisheries and Life Sciences pioneered the application of fish reproductive stem cell culture technology and established for the first time a Chinese hook snout carp spermatogonial stem cell line and a Colia nasus gonadal somatic cell line, which successfully produced sperm after long-term culture in vitro, solving the long-plagued worldwide problem of being unable to culture economic fish reproductive stem cells, and providing a new way for the conservation of endangered fish species, germplasm creation of important economic fishes and gene editing of reproductive stem cells.
This research resolves the problem of inability of long-term culture of fish spermatogonial stem cell lines, and enables spermatogonial stem cells to form sperm in vitro through multi-faceted induction and transplantation studies in vivo and in vitro. This provides a basis for further research and understanding of spermatogonial stem cells, as well as new ideas for protecting endangered species of fish and optimizing ways to breed economically important fish. In the future, Li Mingyou and Guilang's team will combine various research tools such as stem cell culture and induction techniques, gene editing techniques and cell transplantation techniques to establish reproductive stem cell systems for difficult-to-reproduce, long-reproductive-cycle, rare or endangered fishes, and carry out new technologies for high-quality germplasm creation in cultured fish mediated by gene editing of reproductive stem cells.